Fig 1: Smarcd2 is required for chromatin reprogramming after fertilization.(A) Rank plot showing the translation level of the top 1000 translationally up-regulated mRNAs in zygotes. (B) Bar graph showing transcriptional and translational level (RPKM) of Smarcd2 in both MII oocytes and zygotes. (C) Representative images of immunostaining of Smarcd2 in MII oocytes and zygotes. Scale bar, 20 μm. (D) Bar graph showing the relative mRNA level of Smarcd2 in siControl and siSmarcd2 GV oocytes after 24 hours of arrest. (E) Immunostaining showing the reduced Smarcd2 protein level by siRNA in zygotes at 6 hpf. Scale bar, 20 μm. The right panel is the quantification of the Smarcd2 protein level. The average intensity of siControl zygotes were set as 1.0. Each dot represents a single embryo analyzed. ***P < 0.001. (F) Line chart showing the percentage of embryos reaching the indicated stages. The numbers of embryos examined were 44 (siControl), 45 (siSmarcd2#1), and 42 (siSmarcd2#2). (G) Heatmap showing the lost and gained ATAC peaks in siSmarcd2 versus siControl zygotes. Each row represents a locus (ATAC-seq peak center ± 2.5 kb), and the red gradient color indicates the ATAC-seq signal intensity. (H) Metaplot showing the ATAC-seq signals at the TSS of genes with loss of ATAC peaks in siSmarcd2 zygotes.